Biohazard Cabinet Manual NSF Std 49 (92) 011130BIOHAZARDCABINETMANUALModel 10448 - EBC 47, 4-Foot Type A/B3Model 10276, 6-Foot Type AModel 10451, 6-Fo
10Airflow10448 - EBC 47, 4-Ft Type A/B3:Direct Inflow Method Type A/B3-Velocity 100 – 110 fpm-Airflow 253 – 278 cfmCalculated Face Velocity 115 – 125
11NOTES:1. In performing the velocity profile test, the front row of velocity readings(behind the window) must be taken with the thermoanemometer pro
12VII. INSTALLATIONCabinet LocationThe cabinet should be placed in an area of the laboratory free from strong aircurrents, heavy traffic and particul
13Service ConnectionMake sure that a 20 amp, 115 V outlet with the proper receptacle is available for the10448 - EBC 47, 4-Ft Type A/B3. Both the 102
14External Exhaust Connection (Optional)Under normal conditions, the Class II Type A Cabinet does not require externalexhaust from the laboratory. Ho
1510” diameter centered on thimbleTop View10451, 6-Ft Type B3Top View10448, 4-Ft Type A/B3Figure 6: Thimble Exhaust as Viewed from Front of Unit20.87”
16VIII. TESTING AND CERTIFICATIONThe ENVIRCO Biohazard Cabinet has been fully tested by the manufacturer prior toshipment, however, shipping and hand
17Re-certificationIt is recommended that the ENVIRCO Biohazard Cabinet be re-certified every six(6) months and the HEPA filters are replaced if necess
18Testing1. Calibrate the photometer with the aerosol generator so that the upstreamconcentration is 100 percent. The upstream concentration for all
19Drawing NumberPD – 10448-EBC 47WIRING DIAGRAM SIDE SECTION FRONT ELEVATION (Access Panels Removed for Clarity)PICTORIALIX
2CAUTIONS AND WARNINGSREAD AND SAVE THESE INSTRUCTIONSWARNING!TO REDUCE THE RISK OF FIRE, ELECTRICAL SHOCK, OR INJURY TO PERSONS,OBSERVE THE FOLLOWING
20Drawing NumberPD – 10276 / 10451WIRING DIAGRAM SIDE SECTION FRONT ELEVATION (Access Panels Removed for Clarity)PICTORIA
21XI. FILTER REPAIRSCaution: If the cabinet has been used with infectious agents, decontaminationis necessary before removing access panels.Shipping
22XII. AIRFLOW ADJUSTMENTAirflow adjustments can be made with either the solid state speed control inside theelectrical control panel and/or with the
23Rear of work deckProbe set at the bottom edge of the windowFigure 9: Downflow Velocity Grid, Models 10276, 6-Ft Type A and 10451,6-Ft Type B3Inflo
24Exhaust Velocity Grid10448 EBC 47, 4-Ft Type A/B3 Exhaust Velocity Grid10276, 6-Ft Type A10451, 6-Ft Type B3Figure 10: Exhaust
25Electrical TestsElectrical grounding tests should be made to assure that all exposed metal surfacesof the cabinet are grounded.Equipment0-1 ohm full
26Surface MaintenancePainted surfaces and the outside of the safety viewscreen may be cleaned withsoap and water. A light abrasive soap can be used o
27Working in the Biohazard CabinetCaution: Because of the highly specialized nature of the ENVIRCO BiohazardCabinet, it is recommended that an Authori
28XV. CABINET PRESSURE TESTINGThe ENVIRCO Biohazard Cabinet is designed so that there are no contaminatedplenums under positive pressure when the cab
29XVI. LABELING USED ON THE CABINETReview all labeling on the ENVIRCO Biohazard Cabinet as it provides both theinstaller and the worker with importan
3E. Caution: Do not, under any circumstances, cut or remove the grounding prong from theplug.5-20 PPlug5-20 RReceptacle5-30 PPlug5-30 RReceptacle1044
30XVII. WIRING DIAGRAMSFigure 11: 10448 - EBC 47 (4-Ft Type A/B3) Wiring DiagramM1 6 5 4 3 2 1 1 2 3 3 AMPS MAXCONN 'G'
31Figure 12: 10276 (6-Ft Type A) and 10451 (6-Ft Type B3) Wiring DiagramM1 6 5 4 3 2 1 1 2 3 3 AMPS MAX'E1' 'C&apos
32XVIII. HEPA FILTER REPLACEMENTThe HEPA filters require replacement when the air resistance is such that thespecified air velocities cannot be achie
33XIX. REPLACEMENT AND OPTIONAL PARTSPART NUMBERDESCRIPTION 10448 – EBC 47 10276 / 10451Assembly, Motor/Blower 23002 22425 (2 ea.)Motor, 3/4 H.P. O.D
34XX. BIBLIOGRAPHY1. Effective Use of the Laminar Flow Biological Safety Cabinet. U.S. Departmentof Health, Education and Welfare, Office of Biohaz
35XXI. GLOSSARYAerosol: A colloid of liquid or solid particles suspended in a gas, usually air.Ambient: Surrounding on all sides.Biohazard: A cont
36XXII. RISK LEVELS, CLASSIFICATIONLow RiskThe risk level of the agents and/or the operation has minimal effect on personnel,animals, or plants under
37XXIII. MANUFACTURER'S LIMITED WARRANTYThe Biohazard Cabinet is manufactured by ENVIRCO, of Albuquerque, New Mexico andis warranted to be free f
4TABLE OF CONTENTSCAUTIONS AND WARNINGS...2TABLE OF CO
5I. INTRODUCTIONInvestigators and technicians concerned with microbiological safety have for manyyears utilized specialized containment enclosures to
6Class II, Type A/B3 Cabinets:1. Meets the requirements of Class II, Type A cabinets when the HEPA filteredexhaust air is recirculated back to the la
7IV. FEATURES1. The hinged viewscreen is constructed with safety glass and has a seven-degree angle for comfortable viewing. The hinge enables easy
8V. CABINET DIMENSIONSModel A B C D E F10448 – EBC 47, 4-Ft Type A/B3 47.0” 32.5” 62.0” 58.0” 4.0” 28.5”10276, 6-Ft Type A10451, 6-Ft Type B370.0” 32
9VI. SPECIFICATIONSElectrical:10448 - EBC 47, 4-Ft Type A/B3: 115v, 60 Hz, 20 amp10276, 6-Ft Type A: 115v, 60 Hz, 30 amp10451, 6-Ft Type B3: 115v, 60
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